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Gene expression patterns in D-mBMDS cells (high glucose, HG) and undifferentiated mBMDS cells (low glucose, LG). Total RNA was isolated from cultures of undifferentiated mBMDS (grown in LG medium for 4 months) and D-mBMDS (grown in HG medium for 4 months) cell cultures. RT-PCR was performed to detect genes related to β-cell development and insulin production. All PCR products were verified by DNA sequence analysis. The results were repeated at least three times.
