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(A) To create potential DAG sensors, we inserted the cpGFP from G-GECO into the region interconnecting the pseudo substrate and the C1 domain, or in the hinge that connects the C1 domain with the enzyme (red). Some constructs were created with the entire PKCδ, in others the C2 domain was removed. (B) To pair the Upward or Downward DAG sensors with R-GECO, we connected the two coding regions, in frame, with an intervening 2A peptide sequence of 17 amino acids.
