Table 1. Summary of constructs created and tested.
| Sensor | FP position | Truncation site | Deletion | Sensor | FP position | Truncation site | Deletion |
| PcpG1 | C280 | PcpG16 2B | D217 | L122 | |||
| PcpG2 | I282 | PcpG17 2A | N158 | L91 | |||
| PcpG3 | L286 | Upward DAG | N158 | L122 | |||
| PcpG4 | A290 | PcpG17 2C | N158 | L106 | |||
| PcpG5 | Q296 | PcpG17 2D | N158 | Q129 | |||
| PcpG6 | S302 | PcpG17 2A | N158 | K138 | |||
| PcpG7 | E308 | PcpG18 2B | K157 | L122 | |||
| PcpG8 | Y313 | PcpG19 2B | I156 | L122 | |||
| PcpG9 | T320 | PcpG20 2B | Y155 | L122 | |||
| PcpG10 | E325 | PcpG21 2B | H154 | L122 | |||
| PcpG11 | G332 | PcpG22 2B | I153 | L122 | |||
| PcpG12 | I337 | Upward DAG | K152 | L122 | |||
| PcpG13 | K343 | PcpG24 2B | H159 | L122 | |||
| PcpG14 | N348 | PcpG25 2B | E160 | L122 | |||
| PcpG15 | Y448 | PcpG26 2B | F161 | L122 | |||
| PcpG16 | D217 | PcpG27 2B | I162 | L122 | |||
| PcpG17 | N158 | PcpG28 2B | A163 | L122 | |||
| PcpG1 2B | C280 | L122 | PcpG29 2B | T164 | L122 | ||
| PcpG2 2B | I282 | L122 | PcpG30 2B | E134 | L122 | ||
| PcpG3 2B | L286 | L122 | PcpG1-2 | C280 | G281-I282 | ||
| PcpG4 2B | A290 | L122 | PcpG1-3 | C280 | G281-L286 | ||
| PcpG5 2B | Q296 | L122 | PcpG1-4 | C280 | G281-A290 | ||
| PcpG6 2B | S302 | L122 | PcpG1-5 | C280 | G281-Q296 | ||
| PcpG7 2B | E308 | L122 | PcpG1-6 | C280 | G281-S302 | ||
| PcpG8 2B | Y313 | L122 | PcpG1-7 | C280 | G281-E308 | ||
| PcpG9 2B | T320 | L122 | PcpG1-8 | C280 | G281-Y313 | ||
| PcpG10 2B | E325 | L122 | PcpG1-9 | C280 | G281-T320 | ||
| PcpG11 2B | G332 | L122 | PcpG1-10 | C280 | G281-E325 | ||
| PcpG12 2B | I337 | L122 | PcpG1-11 | C280 | G281-G332 | ||
| PcpG13 2B | K343 | L122 | PcpG1-12 | C280 | G281-I337 | ||
| PcpG14 2B | N348 | L122 | PcpG1-13 | C280 | G281-K343 | ||
| PcpG15 2B | Y448 | L122 | PcpG1-14 | C280 | G281-N348 |
The sequence encoding the circularly permuted fluorescent protein was inserted into the PKCδcoding region such that fusions occurred just following the amino acid in PKCδ listed. The N-terminus of PKCδ was truncated in some constructs, with the translation start beginning just before the amino acid listed.