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. 2012 Aug 17;7(8):e43543. doi: 10.1371/journal.pone.0043543

Figure 2. Detection of the kdr mutation by PCR-RFLP.

Figure 2

PCR were conducted with the CpNa-F and CpNa-R primers (Table 3), and then digested with Tsp509I (700 IRDye labelled CpNa-F primer). Lengths of the restricted fragments were determined by electrophoresis in a Li-Cor 4200 automatic DNA sequencer (6.5% polyacrylamide denaturing gel) and visualised using the SAGA software (Li-Cor Biosciences). The 77 bp fragment corresponds to the kdr allele. The 101 and 112 bp fragments correspond to two different susceptible alleles. A–F corresponds to the different PCR-RFLP genotypes: 77/77, 101/101, 112/112, 77/101, 101/112 and 77/112. G is the length of the non-restricted PCR product (170 bp). 50–350 pb sizing standard (Biosciences) is in the left well.