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The splicing patterns of U12 intron-containing transcripts were analyzed by RT-PCR in wild type (WT), AtU11/U12-31K knockdown mutant (amiR1-4), and complementation lines expressing OsU11/U12-31K (amiR1-4/Os31K). Identical results were obtained from three independent experiments, one of which is shown. Expression of OsU11/U12-31K in each complementation line (amiR1-4/Os31K) was confirmed by RT-PCR.
