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. 2012 Aug 17;7(8):e43707. doi: 10.1371/journal.pone.0043707

Figure 7. RNA chaperone activity of OsU11/U12-31K protein.

Figure 7

(A) The colony-forming abilities of diluted BX04 E. coli cells expressing either OsU11/U12-31K (Os31K), CspA (positive control), or pINIII (negative control) were examined under normal (37°C) and cold stress (20°C) conditions. (B) The DNA-melting activity of OsU11/U12-31K protein was examined by monitoring the fluorescence of a 78-nucleotide-long molecular beacon with the addition of GST-Os31K (10 µg), GST-CspA (5 µg), or GST (5 µg). (C) Enhanced RNase T1 cleavage of the substrate RNA (R) was measured after the addition of OsU11/U12-31K protein (GST-31K). The RNase T1-resistant bands, as indicated by arrows, disappeared in the presence of recombinant GST-31K fusion protein. The numbers above each column represent the amount of T1 added to the reaction.