A & B, Digestive tissue cDNA panel. C & D, Fetal and Adult tissue cDNA panels. The primer pairs used were as for the prostate and fetal brain analysis (Figure 1). There was little or no amplification following 1°PCR, but 2° PCR resulted in definite bands. The number of 2° PCR cycles is shown on the left of each set. The results show that only V4 amplified; V1 and V5 transcripts were absent. Digestive tissue panel:
C, cecum; AC, ascending colon; DC, descending colon; TC, transverse colon; D, duodenum; E, esophagus; Ilc, ileocecum; Ile, ileum; J, jejunum; L, liver; R, rectum; St, stomach. Fetal and adult tissue panel: B, brain; H, heart; K, kidney; L, liver; Lu, lung; P, pancreas; Pl, placenta; S, skeletal muscle; Sp, spleen; T, thymus.