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. 2012 Aug 17;7(8):e43174. doi: 10.1371/journal.pone.0043174

Figure 4. Amplification of V1 with unique V1-specific antisense primers.

Figure 4

The sense primer was the same as for primary PCR of other variants, but the antisense primers were specific for the 108-bp segment absent in V4, as well as other variants. Because the amplification was quite robust upon primary PCR, nested PCR was not required. A, Digestive tissue panel: C, cecum; AC, ascending colon; DC, descending colon; TC, transverse colon; D, duodenum; E, esophagus; Ilc, ileocecum; Ile, ileum; J, jejunum; L, liver; R, rectum; St, stomach. B, Fetal and adult tissue panels: B, brain; H, heart; K, kidney; L, liver; Lu, lung; P, pancreas; Pl, placenta; S, skeletal muscle; Sp, spleen; T, thymus.