Figure 2. CD4⁺ T cells reduce the burden of virulent Salmonella infection.

C57BL/6 mice were infected orally with 1×10⁷ virulent Salmonella Typhimurium. (A) At day 7, 14, 21 and 28 post-infection, the Salmonella CFU in the spleen were determined. Data show the mean ± SEM of 5–7 mice per timepoint. (B) At the onset of Salmonella infection, CD4⁺ or CD8⁺ T cell populations were depleted with anti-CD4 (clone GK1.5) or anti-CD8 (clone 2.43) monoclonal antibodies and at day 14 post-infection recoverable Salmonella CFU were determined. Graph shows mean + SEM for 5–6 mice per group. Statistical significance between groups is indicated (Mann-Whitney test, *p<0.05). Experiments were performed twice with similar results.