Table 1. CD4+ T cell epitopes to virulent Salmonella.
Protein | Epitopea | Sequence | Protein function | ELISPOTb |
AhpC | 154–168 | AAQYVAAHPGEVCPA | alkyl hydroperoxide reductase subunit C | 5.1 |
EutC | 243–257 | CYAVYSPRVATTVEA | ethanolamine ammonia-lyase small subunit | 2.4 |
STM1540 | 262–276 | GVYYTTYAPQATSAH | putative hydrolase | 17.4 |
Indicated is the amino-acid position in Salmonella Typhimurium strain LT2 [28].
Mice were infected orally with 1×107 Salmonella Typhimurium (strain LT2). After 18 days, CD4+ T cells were tested in ELISPOT assays for IFN-γ production. Data is presented as Stimulation Index (SI), calculated as the number of spot forming cells after stimulation with peptide-loaded dendritic cells divided by spot forming cells in background.