Figure 3. Neuronal differentiation of newly born cells in the subgranular zone-granule cell layer (SGZ-GCL). Analyses were performed at 24 hours after the last of twelve daily injections of 5′-bromodeoxyuridine (BrdU) and visualized through BrdU and doublecortin (DCX) dual immunofluorescence and confocal microscopy.

Figures A1–C1 show examples of newly born (BrdU+) cells (red nuclei) that are positive for DCX (green fluorescence in the cytoplasm of the soma and dendrites). The bar chart in D1compares percentages of BrdU+ cells that express DCX (n = 5/group). Note that AS activity diminishes the overall neuronal differentiation of newly born cells. Scale bar, A1–B3 = 20 µm.