Figure 2. The p53^R72 polymorphic allele has increased ability to target OKL38 to mitochondria than the p53^P72 allele.

(A) p53^P72 (green) and OKL38 (pseudocolored blue) localized at the nucleus in Saos-2 cells at 39°C. (B) p53^R72 (green) and OKL38 (blue) localized at the nucleus in Saos-2 cells at 39°C. (C) p53^P72 (green) and OKL38 (blue) still localized at the nucleus in Saos-2 cells after temperature shift to 32°C. (D) p53^R72 (green) and OKL38 (blue) colocalized with the staining of MitoTracker Red, a mitochondria dye, in Saos-2 cells after temperature shift to 32°C. (E) Percentages of cells with nuclear or mitochondrial localization of p53 and OKL38 were analyzed from three independent experiments. Standard deviations are shown (n = 3).