Table 1. Candidate Dgcr8-independent miRNAs.
Dgcr8 independent miRNA candidates | Enzymatic Dependency observed | Fold Change | Still in miRBase v18 | Reason cited in miRBase for removal* |
mmu-miR-1186 | This study | 0.985 | Yes | |
mmu-miR-2142 | This study | 0.950 | No | “fragment of 5S rRNA” This study |
mmu-miR-805 | This study | 1.186 | No | “overlaps a Mt tRNA” |
mmu-miR-1274a | This study | 0.948 | No | “fragment of a Lys tRNA (Schopman et al.)1” |
mmu-miR-1937a | This study | 1.500 | No | “fragment of tRNA” |
mmu-miR-1937b | This study | 1.516 | No | “fragment of tRNA” |
mmu-miR-2132 | This study | 1.174 | No | “fragment of rRNA (Chiang et al.)2” |
mmu-miR-2143 | This study | 1.447 | No | “fragment of 28S rRNA” |
mmu-miR-2141 | This study | 0.897 | No | “fragment of rRNA (Chiang et al.)2” |
mmu-miR-720 | Babiarz et al. | 1.178 | Yes | |
mmu-miR-1983 | Babiarz et al. | 4.324 | Yes | |
mmu-miR-320 | Babiarz et al. | 2.172 | Yes | |
mmu-miR-122 | Babiarz et al. | 0.519 | Yes | |
mmu-miR-484 | Babiarz et al. | 1.581 | Yes | |
mmu-miR-1981 | Babiarz et al. | 5.821 | Yes | |
mmu-miR-344 | Babiarz et al. | 0.645 | Yes | |
mmu-miR-877 | Babiarz et al. | 3.105 | Yes | |
mmu-miR-668 | Babiarz et al. | 0.688 | Yes | |
mmu-miR-702 | Babiarz et al. | 1.421 | Yes | |
mmu-miR-1982 | Babiarz et al. | 0.995 | Yes | |
mmu-miR-689 | Babiarz et al. | 0.631 | No | “fragment of rRNA (Chiang et al.)2” |
mmu-miR-699 | Babiarz et al. | NA | No | “fragment of RNase MRP RNA” |
Presented are the candidate Dgcr8-independent miRNAs alongside those identified as Dgcr8-independent by Babiarz et al. [25]. Included is the fold change of each miRNA seen in this study between heterozygous and Dgcr8-depleted cell lines (bold italics if they meet our fold change threshold) and the status of each miRNA in miRBase version 18.
Reasons for removal from miRBase (http://www.mirbase.org/) as given in miRBase, except for miR-2142, which was identified for removal by this study (1 [42], 2 [41]).