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. 2012 Aug 17;7(8):e43208. doi: 10.1371/journal.pone.0043208

Figure 3. Cdc25 is essential for the response to arsenate.

Figure 3

A. Arsenate to arsenite conversion in fission yeast. Cell extracts from cells treated with 100 µM sodium arsenate were analyzed for the presence of As (III) at different time points. Graph represents parts per million (ppm) As (III). B. Protein alignment of a fragment of S. pombe Cdc25, rice Cdc25 and S. cerevisiae Acr2 and human Cdc25. Asterisks indicate full conservation. C. Serial dilutions of wild type,cdc2-3w and cdc2-3w cdc25Δ strains were plated in rich media (YES) or rich media containing 25 µM sodium arsenate. Pictures were taken after incubation at 30°C for 48 hours. D. Western blotting of whole cell extracts from Cdc25:myc strains treated with 100 µM sodium arsenate for 0 to 180 minutes. Anti-myc antibodies were used to detect Cdc25:myc and anti-actin as a control. E. Total RNA from the experiment presented in (D) was purified and the total amount of Cdc25 mRNA quantified by qPCR. Actin mRNA was used as an internal control.