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A:Western blot analysis of ERK ½ phosphorylation (pERK) as compared to total ERK ½ (tERK) is increased upon treatment with 1 nM ET-1 in cultured trabeculae. B: Increases in the phospho-ERK1/2 expression in ET-1 treated muscles are augmented by PD98095, Akt inhibitor and 2-APB. Values are mean ± SEM from 4–7 muscles in each group. *P<0.05 vs. control by student t-test.
