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. 2012 Aug 17;7(8):e43204. doi: 10.1371/journal.pone.0043204

Figure 1. HBx-d382 enhances the non-anchored growing ability of transfected hepatocytes.

Figure 1

(A–B) The identification of stable HBx transfection in LO2 cells. (A) The HBx gene was identified by RT-PCR. (B) Western blotting showed the expression of HBx in LO2 cells. 1: LO2; 2: LO2/pcDNA3.0; 3: LO2/HBx-d382; 4: LO2/HBx; M: marker. (C) Soft agar colony formation assay of transfected LO2 cells. The rate of colony formation in HBx-expressing cells was significantly higher than in control LO2 and LO2/pcDNA3.0 cells (*P<0.01). While within the groups of LO2/HBx-d382 LO2/HBx, the former was higher than the latter (#P<0.01).