|
Photorhabdus luminescens strains |
|
|
| LN2/LN2-W |
Wild-type isolate from host nematode H. indica LN2 |
Ralf-Udo Ehlers |
| LN2-R1∼LN2-R34 |
Spontaneous RifR mutant of wild-type strain LN2; RifR
|
This study |
| LN2-A |
Spontaneous AmpR mutant of wild-type strain LN2; AmpR
|
This study |
| LN2-M1 |
Tn5 insertion mutant of LN2; AmpR, KmR
|
[41]
|
| H06 |
Wild-type isolate from host nematode H. bacteriophora H06 |
Laboratory stock |
| HNA |
Wild-type isolate from host nematode H. megidis HNA |
Dr Wim Wouts |
| LN2-WΔrpoB-LR31 |
LN2-W containing the mutant rpoB allele from LN2-R31 |
This study |
| LN2-R31ΔrpoB-LW |
LN2-R31containing the wild-type rpoB allele from LN2-W |
This study |
| LN2ΔrhlE
|
LN2-A rhlE::Cm |
This study |
| LN2ΔnamB
|
LN2-A namB::Cm |
This study |
| LN2ΔdsbA
|
LN2-A dsbA::Cm |
This study |
|
Escherichia coli strains |
|
|
| DH5a |
Host of plasmids |
TaKaRa |
| S17-1 (λpir) |
E. coli lysogenized with λpir, replication of ori R6K |
[51]
|
| TOP10 |
Cloning strain |
Invitrogen |
| Plasmids |
|
|
| pMini-Tn5
|
oriT, oriR6K, delivery plasmid for mini-Tn5; KmR
|
[52]
|
| pCR4-TOPO |
Cloning vector; AmpR, KmR
|
Invitrogen |
| pMD-18T |
Cloning vector; AmpR
|
TaKaRa |
| pMD-19T |
Cloning vector; AmpR
|
TaKaRa |
| pUC19-egfp |
pUC19 carrying egfp; AmpR
|
Laboratory stock |
| pMD-egfp |
pMD-18T carrying ribosome binding site and egfp gene; AmpR
|
This study |
| pMD-lac-egfp |
pMD-19T carrying ribosome binding site, lacZ promoter and egfp gene; AmpR
|
This study |
| pMini-lac-egfp |
pMini-Tn5 carrying a NotI fragment containing ribosome binding site, lacZ promoter and egfp gene; KmR
|
This study |
| pPHU281 |
lacZ’ mob(RP4), TcR derivative of pUC18 with oriT
|
[50]
|
| pPHU281-rpoB-LW |
pPHU281 carrying a BamHI-PstI fragment containing rpoB gene from wild-type strain of P. luminescens LN2 |
This study |
| pPHU281-rpoB-LR31 |
pPHU281 carrying a BamHI-PstI fragment containing rpoB gene from RifR mutant of P. luminescens LN2-R31 |
This study |
| pKNG101 |
R6K Ori, sacB, SmR
|
[56]
|
| pKNG101-rhlE::Cm |
pKNG101 carrying a rhlE::Cm fragment, SmR, CmR
|
This study |
| pKNG101- namB::Cm |
pKNG101 carrying a namB::Cm fragment, SmR, CmR
|
This study |
| pKNG101- dsbA::Cm |
pKNG101 carrying a dsbA::Cm fragment, SmR, CmR
|
This study |
