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. 2012 Aug 17;7(8):e43114. doi: 10.1371/journal.pone.0043114

Table 3. Total proteins with altered level of synthesis in the nematicidal-producing and non nematicidal-producing mutants.

Spot No. Protein Name/plu Organism Gene Function Protein PI Protein MW Ratio
W/W R2/W R16/W R31/W R33/W
Ribosomal protein
6 30S ribosomal protein S8 P. luminescens TT01 rpsH Binds directly to 16S rRNA central domain where it helps coordinate assembly of the platform of the 30S subunit 9.35 14205.6 1 0.7 0.8 0.27 1.03
15 50S ribosomal protein L17, plu4701 P. luminescens TT01 rplQ A component of the macrolide binding site in the peptidyl transferase cente 11.04 14708.8 1 0.5 1.27 0.69 1.34
26 50S ribosomal protein L3, plu4726 P. luminescens TT01 rplC Binds directly near the 3′ end of the 23S rRNA, where it nucleates assembly of the 50S subunit; essential for peptidyltransferase activity; mutations in this gene confer resistance to tiamulin 9.87 22328.9 1 0 0.41 0 0
44 50S ribosomal protein L9 plu4570 P. luminescens TT01 rplI In E. coli this protein is wrapped around the base of the L1 stalk 6.13 15872.5 1 2.38 2.23 0.92 1.37
45 50S ribosomal protein, L9 plu4570 P. luminescens TT01 rplI In E. coli this protein is wrapped around the base of the L1 stalk 6.13 15872.5 1 1.82 3.29 0.51 1.23
Adaptations conditions
7 Hypothetical protein, plu2032 P. luminescens TT01 Similar to Unknown protein YbdQ of E. coli; Similar to universal stress protein (pfam00582: Usp) 6.19 15907.4 1 3.79 2.9 1.46 4.83
9 Hypothetical protein, plu2030 P. luminescens TT01 Similar to Unknown protein YbdQ of E. coli, Similar to universal stress protein (pfam00582: Usp) 5.76 15283.2 1 1.71 1.21 0.2 0.45
48 Alkyl hydroperoxide reductase, small subunit (antioxidant), plu3907 P. luminescens TT01 ahpC Alkyl hydroperoxide reductase, small subunit (antioxidant) 5.98 22259.3 1 0.8 0.82 0.9 0.47
Secondary metabolites
12 Crystalline inclusion protein CipB P. luminescens TT01 cipB Unknown, similar to crystalline inclusion protein type II 6.08 11281.6 1 0.12 0 0.51 0
25 Crystalline inclusion protein CipA, plu1576 P. luminescens TT01 cipA Crystalline inclusion protein CipA 6.06 11710.9 1 0 0.08 0.74 0
32 Unknown P. luminescen Similar to hemolysin from Fusobacterium nucleatum clinical isolate found in GenBank, Accession Number AF525507 5.61 39995.2 1 0.9 1.7 0.44 0.8
62 Hypothetical protein, plu4211 P. luminescens TT01 Highly similar to Hcp protein 6.29 18482.5 1 11.43 14.91 19.98 3.24
Metabolism of amino acids and related molecules
30 Ethanolamine ammonia-lyase small subunit, plu2971 P. luminescens TT01 eutC Catalyzes the formation of acetaldehyde from ethanolamine 6.37 31188.4 1 4.17 0.5 1.1 1.2
36 Tryptophan synthase subunit beta, plu2466 P. luminescens TT01 trpB Catalyzes the formation of L-tryptophan from L-serine and 1-(indol-3-yl)glycerol 3-phosphate 6.2 43098 1 1.22 0.25 0.41 0.58
46 Serine/arginine repetitive matrix protein 2 Srrm2 12.02 294500.8 1 0.72 1.7 0.89 0.9
50 2,3-dihydroxy-2,3-dihy drophenylpropionate dehydrogenase, plu2207 P. luminescens TT01 hcaB Converts cis-3-(3-carboxyethyl) -3,5-cyclohexadiene-1,2-diol (PP-dihydrodiol) into 3-(2,3-dihydroxylphenyl) propionate 5.43 29519.1 1 0.37 0.3 0.49 0.9
51 Hypothetical protein, plu4676 P. luminescens TT01 Similar to 3-oxoacyl-[acyl-carrier-protein] synthase II (beta-ketoacyl-ACP synthase II) (KAS II) 5.58 45407.3 1 3.29 3.0 0.01 <0.01
52 Serine hydroxymethyltransferase, plu3291 P. luminescens TT01 glyA Catalyzes the reaction of glycine with 5,10-methylenetetrahydrofolate to form L-serine and tetrahydrofolate” 5.92 45229.8 1 3.74 4.55 2.93 5.6
53 Urease accessory protein, plu2176 P. luminescens TT01 ureG Urease accessory protein 5.04 22868.1 1 4.42 2.98 5.17 6.66
54 Hypothetical protein, plu2040 P. luminescens TT01 Similar to vibrio bactin utilization protein ViuB 5.51 31363 1 2.36 3.61 1.83 6.57
55 3-oxoacyl-(acyl carrier protein) synthase III, plu2835 P. luminescens TT01 fabH FabH; beta-ketoacyl-acyl carrier protein synthase III; catalyzes the condensation of acetyl-CoA with malonyl-ACP to initiate cycles of fatty acid elongation; differs from 3-oxoacyl-(acyl carrier protein) synthase I and II in that it utilizes CoA thioesters as primers rather than acyl-ACPs” 5.33 34189.6 1 5.12 6.14 3.85 6.77
Nucleosides and nucleotides biosynthesis and metabolism
1 Hypothetical protein, plu3994 P. luminescens TT01 Similar to putative membrane protein YqjD (carboxyl transferase) of E. coli 7.93 11042 1 4.03 0.57 2.91 1.2
3 IS630 family transposase, plu0720 P. luminescens TT01 ISPlu3Y Transposase, IS630 family 9.35 39771.5 1 0.38 0.17 0.02 0.11
47 IS630 family transposase, plu0468 P. luminescens TT01 ISPlu10J Transposase 9.59 39687.9 1 0.85 0.6 0.71 0.25
4 ATP-dependent RNA helicase RhlE, plu1511 P. luminescens TT01 rhlE This helicase is not essential cell growth 10.01 48258.8 1 0.05 0.52 0.04 0.02
19 ATP-dependent RNA helicase RhlE, plu1511 P. luminescens TT01 rhlE This helicase is not essential cell growth 10.01 48258.8 1 0 1.43 0.2 0
18 Nucleoside diphosphate kinase, plu1372 P. luminescens TT01 ndk Catalyzes the formation of nucleoside triphosphate from ATP and nucleoside diphosphate 5.35 15591.8 1 1.43 1.96 2.48 2.53
57 Uracil phosphoribosyl transferase, plu2759 P. luminescens TT01 upp Catalyzes the formation of uracil and 5-phospho-alpha-D-ribosy 1-diphosphate from UMP and diphosphate 5.46 22489 1 1.59 2.81 1.83 4.95
58 Reverse gyrase Leptospirillum sp. Group II UBA 9.31 56458 1 0 4.93 6.32 0.41
Cell wall/membrane biogenesis
8 Karst CG12008-PA, isoform A Drosophila melanogaster kst Kst-PA; spectrin beta-heavy chain; beta-H spectrin; 5.93 471351.1 1 2.20 1.8 1.33 1.16
43 hypothetical protein, plu3994 P. luminescens TT01 Similar to putative membrane protein YqjD of E. coli 7.93 11042 1 0.21 0.05 0.1 0.08
5 Periplasmic chaperone, plu0681 P. luminescens TT01 hlpA Histone-like protein HLP-1 precursor 9.43 18476.8 1 2.63 0.81 2.11 2.64
Transport and binding proteins
10 Unknown P. luminescens W14 From (type III secretion system, partial sequence) GI:27550090 6.1 16883.6 1 0 1.53 0 0
64 Unknown P. luminescens w14 From (type III secretion system, partial sequence) GI:27550090 6.1 16883.6 1 0.1 0.91 0.11 0.15
65 Unknown P. luminescens w14 From (type III secretion system, partial sequence) GI:27550090 6.1 16883.6 1 0.11 0.93 0.11 0.16
11 Hypothetical protein, plu1886 P. luminescens TT01 Hypothetical transmembrane protein 5.88 15588.6 1 1.53 2.83 1.57 0.43
13 Macrolide transporter subunit MacA, plu1590 P. luminescens TT01 macA Probable macrolide-specific ABC transporter; confers macrolide resistance via active drug efflux 6.95 40743.2 1 0 0 0 0
17 Sec-independent protein translocase protein, plu4410 P. luminescens TT01 tatA Sec-independent protein translocase protein 6.18 9289.9 1 0.76 0.81 0.72 0.23
42 Na-binding protein HU-alpha (NS2) (HU-2), plu0492 P. luminescens TT01 dbhA Na-binding protein HU-alpha (NS2) (HU-2) 9.1 9407 1 2.1 2.3 0.59 0.99
Information and regulatory pathways
14 DNA-binding transcriptional regulator HexR, plu2121 P. luminescens TT01 hexR Represses the expression of the zwf, eda, glp and gap 6.97 31694.5 1 2.2 1.96 1.92 0.91
16 DnaK transcriptional regulator DksA, plu0876 P. luminescens TT01 dksA DnaK transcriptional regulator DksA 5.04 17415.8 1 0 0.05 0.65 0
20 Nucleotide-binding protein, plu3881 P. luminescens TT01 Similar to Unknown protein YajQ of E. coli 5.77 18311.4 1 1.47 0.89 1.48 1.09
22 Transcriptional repressor MprA, plu1277 P. luminescens TT01 mprA DNA-binding transcriptional repressor of microcin B17 synthesis and multidrug efflux; negative regulator of the multidrug operon emrAB 7.01 20365.5 1 1.67 1.18 1.38 1.09
27 Hypothetical protein, plu0318 P. luminescens TT01 Similar to AidA protein of Ralstonia solanacearum 5.7 22068.3 1 1.03 1.68 0.86 0.87
49 Periplasmic protein disulfide isomerase I, plu0381 P. luminescens TT01 dsbA Disulfide interchange protein DsbA precursor 7.7 22954.8 1 3.8 1.57 3.49 3.62
37 Protease precursor DegQ, plu4018 P. luminescens TT01 degQ Protease precursor DegQ 9.12 48028.9 1 0 0 0 0
38 Protease precursor DegQ, plu4018 P. luminescens TT01 degQ Protease precursor DegQ 9.12 48028.9 1 0 0 0 0
Energy production and conversion
24 GD22749 Drosophilia simulans Dism\GD22749 Chromosome segregation ATPases [Cell division and chromosome partitioning]; COG1196 5.23 76326.8 1 1.39 0 <0.01 <0.01
23 Hypothetical protein, plu2075 P. luminescens TT01 Similar to 3-oxoacyl-[acyl-carrier protein] reductase 8.55 25032.6 1 0.12 0.31 0.45 0.33
35 WblA protein, plu4796 P. luminescens TT01 wblA Probable lipopolysaccharide biosynthesis protein; Similar to putative UDP-glucose/GDP-mannose dehydrogenase 5.86 48484 1 2.1 0 2.07 0.05
40 Catalase, plu3068 P. luminescens TT01 katE Catalase 6.92 55509.5 1 0 0 0 0
41 Catalase plu3068 P. luminescens TT01 katE Catalase 6.92 55509.5 1 0 0 0 0
56 Phosphoglycero mutase, plu1471 P. luminescens TT01 gpmA Catalyzes the interconversion of 2-phosphoglycerate to 3-phosphoglycerate 5.62 28396.7 1 1.64 2.88 2.02 3.5
Phage-related proteins
28 Hypothetical protein, plu3012 P. luminescens TT01 Probable phage protein; Similar to tail fiber assembly protein from bacteriophage 4.67 21467.8 1 0.68 0.88 2.36 0.44
29 Hypothetical protein, plu2035 P. luminescens TT01 Some similarities with putative tail fiber protein of prophage 4.23 23641.5 1 0 3 0.42 0.88
39 Hypothetical protein, plu3803 P. luminescens TT01 Some similarities with prophage tail fiber protein 6.37 66850.4 1 0.23 0.5 0.1 0
60 Hypothetical protein, plu3032 P. luminescens TT01 Putative bacteriophage protein; Some similarities with Unknown protein of Photorhabdus 6.08 22021.6 1 1.93 2.55 2.14 4.66
63 Hypothetical protein, plu3012 P. luminescens TT01 Probable phage protein; Similar to tail fiber assembly protein from bacteriophage 4.67 21467.8 1 1.66 1.72 5.27 0.48
Flagellin
31 Flagellin, plu1954 P. luminescens TT01 fliC Structural flagella protein 5.19 38183.6 1 0 0.1 0.87 0
Post-translational modification
61 PTS system, N-acetyl- galactosamine-specific IIB component 2 (EIIB-AGA’) (N-acetyl- galactosamine-perme, plu0835 P. luminescens TT01 agaV Probable PTS system; Highly similar to PTS system, cytoplasmic, N-acetylgalactosamine-specific 6.51 17774.4 1 3.87 2.18 4.31 1.5
Uknown
2 Hypothetical protein, plu0661 P. luminescens TT01 Highly similar to Unknown protein YgdH of E. coli 5.95 51257.2 1 2.74 0.81 2.51 1.76
21 Unknown Fanconi anemia group D1 protein LOC725687 Similar to Breast cancer type 2 susceptibility protein 8.75 82829.5 1 0.05 0 0 0
33 Hypothetical protein, plu3611 P. luminescens TT01 Similar to Unknown protein YggE of E. coli 5.97 25893.5 1 0 2.33 0.2 0.4
34 Hypothetical protein MHP7448_0445 Mycoplasma hyopneumoniae 7448 GI:72080777 9.17 275940.9 1 0.39 0.19 0.1 0.05
59 Hypothetical protein UM02446.1 Ustilago maydis 521 GI:124514614 8.96 130494.3 1 11.46 2.78 6.42 6.6