Figure 6. WA inhibited tumor growth in murine MPM allograft.

Female BALB/c mice bearing AB 12 tumors were treated with either vehicle (V) or WA as described in methods. Animals were monitored every day for tumor volumes (growth) as well as any activity alteration. Tumor volumes were measured every other day for the control and treated groups (A). Points, mean tumor volume in each experimental group containing 4 mice. Bars, SD. Tumors were collected after 17 days of treatment, and the tumor biopsies were analyzed by the proteasomal chymotrypsin-like activity assay (B) and Western blotting for ubiquitinated proteins (Ub-prs) and Bax (C) essentially as detailed in methods. D, The tumor tissues from the control and treated groups were immuno-stained for apoptosis (by TUNEL assay) and levels of CDKI p27, oncogene c-myc and CARP-1 proteins as noted in methods. Magnifications are 400×.