Table 1. Differentially expressed ESTs identified in both libraries based on their enzyme codes.
| Up-regulated genes | ||||||
| Enzyme Codea | Enzyme name | Cellular localization | No. ESTs Control tissue | No. ESTs Inoculated tissue | p-value | REb |
| EC:5.3.1.6 | ribose-5-phosphate isomerase | chloroplast | 0 | 3 | 0.09 | 1.06 |
| EC:3.5.1.1 | asparaginase | unknown | 0 | 3 | 0.09 | 1.06 |
| EC:2.3.1.0 | unknown | membrane | 0 | 3 | 0.09 | 1.06 |
| EC:1.1.1.95 | D-3-phosphoglycerate:NAD+ oxidoreductase | chloroplast, nucleus, cytosol, mitochondria | 0 | 3 | 0.09 | 1.06 |
| EC:3.2.1.39 | endo-1,3- β -glucanase; callase | vacuole, endomembrane system | 1 | 5 | 0.09 | 1.02 |
| Down-regulated genes | ||||||
| Enzyme Code a | Enzyme name | Cellular localization | No. ESTs Control tissue | No. ESTs Inoculated tissue | p-value | RE b |
| EC:4.1.1.39 | ribulose-bisphosphate carboxylase | chloroplast | 144 | 75 | 0.002 | −2.66 |
| EC:2.7.11.25 | MAPKKK; MEKK | cytoplasm, plastid, plasma membrane | 8 | 1 | 0.05 | −1.30 |
| EC:2.1.2.1 | Serine and threonine aldolase | cytosolic ribosome, membrane, apoplast | 5 | 0 | 0.07 | −1.15 |
The enzyme codes were established using KEGG as part of the Blast2GO suite. Statistical significance was calculated with the Fisher’s exact test (p≤0.1).
a
The ESTs names corresponding to enzyme code are described in Table S1.
b
RE = relative expression values were obtained by -Log10 of p-values for the up-regulated transcripts and by Log10 of p-values for the down-regulated genes, according to Zhou et al. [79].