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. 2012 Aug 17;7(8):e43701. doi: 10.1371/journal.pone.0043701

Figure 2. Deletion analysis of the S1CD-interacting domain of Topors.

Figure 2

(A) Model of murine Topors protein domains, with the sequence corresponding to the S1CD-interacting prey 36 sequence identified by a black box. (B) Deletional analysis of the S1CD-binding sequence of Topors. Yeast with either LexA-S1CD or LexA-Lamin bait plasmid were transformed with either prey plasmid for the expression of complete S1CD binding sequence (Topors 398–516), or with prey plasmid expressing deletion mutants of Topors 398–516. After selection of co-transformants, interacting bait-prey pairs were identified by assay of β-galactosidase activity. Yeast two-hybrid analysis of Topors deletion mutants identifies residues 493–510 as critical for the interaction.