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. 2012 Aug 17;7(8):e43098. doi: 10.1371/journal.pone.0043098

Figure 1. Transgene and GFP expression depends on cre in cells and tissues.

Figure 1

(A) Schematic of the transgene construct showing the β-actin promoter (β-actinpro), the stop cassette (STOP) flanked by lox P sites (triangles), the epitope-tagged Wnt9b cDNA (Wnt9bFLU), an internal ribosomal entry site (IRES) and green fluorescent protein cDNA (GFP). (B) Epitope-tagged Wnt9b protein is expressed when the transgene is introduced into Cos-1 cells (lanes 1, 2) and E11.5 transgenic embryos (lanes 3–6) only when cre recombinase is present (cre +). There is a higher molecular weight background band present in the tissue lysates that is not altered by the presence of cre. Lane 7 is empty. A positive control vector that lacks the lox-STOP cassette expresses Wnt9b-FLU in the absence of cre (lane 8). Brightfield (C–D) and fluorescence (E, F) images of Wnt9b transgenic E11.5 embryos with (D,F) and without (C,E) β-actin-cre demonstrate GFP expression and delayed embryogenesis for the Wnt9b tg/+, β-actin-cretg/+ embryos.