Figure 7. Pyloric gene expression exhibits dose-dependent effects in the Wnt9b and stabilized β-catenin alleles.

Whole mount in situ analysis of Grem1, Nkx2.5, and Gata3 expression in E12.5 stomachs. All three genes are restricted to the pyloric sphincter in wild-type stomachs (Six2-cre^tg/+). Grem1 and Gata3 show decreased expression in Wnt9b transgenics (Six2-cre^tg/+, Wnt9b^tg/+) that is further downregulated in the stabilized β-catenin allele (Six2-cre^tg/+, Ctnnb1^ex3/+). Nkx2.5 expression is expanded into the stomach in Wnt9b transgenics and further upregulated in the stabilized β-catenin allele. Nkx2.5 expression that can be seen at the upper right of the stomach is found in the spleen on the opposite side.