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. 2012 Aug 17;7(8):e43607. doi: 10.1371/journal.pone.0043607

Figure 4. Cryo-SEM micrographs of B. nigra leaf surfaces and adhering herbivore eggs and egg – leaf contact regions.

Figure 4

(A–E) abaxial site of B. nigra leaves. (A) Eggs of P. brassicae 72 hao with surrounding leaf surface of HR+ B. nigra and trichomes. (B) Eggs of M. brassicae (48–60 h old) with surrounding leaf surface of HR− B. nigra. (C) Contact region after P. brassicae egg removal (72 hao) on HR− B. nigra consisting of accessory reproductive gland (ARG) secretion functioning as cement (arrow). (D) Contact region after M. brassicae egg removal consisting of a part of ARG cement and healthy leaf cells with open stomata (arrow). (E) Necrotic zone on HR+ B. nigra leaf induced by P. brassicae eggs 72 hao, with some eggs removed. ARG-derived cement is removed together with parts of the cell layer (arrow) and open stomata at the border between dead and living cells. (F) Adaxial site of necrotic zone of HR+ B. nigra with open stomata (arrow) between healthy cells and necrotic zone on right side. Hypersensitive response type, HR−: no necrotic zone observed, HR+: with necrotic zone. Scale bars are given in lower right corners.