Table 3. Proteins from each of the Top 3 most significantly dysregulated pathways identified by SILAC and subsequent IPA-Tox analysis of WM1158 cells treated with Elesclomol (E) or the drug vehicle DMSO (V).
| Symbol | Entrez Gene Name | UniProt | Peptide Count | Fold-reduction in E- versus V-treated cells |
| Mitochondrial Dysfunction | ||||
| CPT1A | Carnitine palmitoyltransferase 1A | P50416-1 | 6c | 2.6 |
| NDUFA7 | NADH dehydrogenase (ubiquinone) 1 alpha subcomplex 7, 14.5 kDa (cmplx I) | O95182 | 9u | 2.1 |
| NDUFA2 | NADH dehydrogenase (ubiquinone) 1 alpha subcomplex 2, 8 kDa (cmplx I) | O43678 | 4u | 2.0 |
| NDUFA10 | NADH dehydrogenase (ubiquinone) 1 alpha subcomplex 10, 42 kDa (cmplx I) | O95299 | 2u+8c | 1.9 |
| PRDX5 | Peroxiredoxin 5 | P30044-1 | 26c | 1.8 |
| SDHA | Succinate dehydrogenase complex, subunit A, flavoprotein (cmplx II) | B3KYA5 | 5c | 1.7 |
| CYCS | Cytochrome c, somatic | P99999 | 4c | 1.7 |
| NDUFB11 | NADH dehydrogenase (ubiquinone) 1 beta subcomplex 11, 17.3 kDa (cmplx I) | Q9NX14-1 | 4c | 1.6 |
| CASP3 | Caspase 3, apoptosis-related cysteine peptidase | P42574 | 10c | 1.6 |
| NDUFA4 | NADH dehydrogenase (ubiquinone) 1 alpha subcomplex 4, 9 kDa (cmplx I) | O00483 | 13u | 1.6 |
| NDUFS8 | NADH dehydrogenase (ubiquinone) Fe-S protein 8, 23 kDa (cmplx I) | O00217 | 2c | 1.6 |
| SOD2 | Superoxide dismutase 2, mitochondrial | P04179 | 13c | 1.5 |
| NDUFB4 | NADH dehydrogenase (ubiquinone) 1 beta subcomplex 4,15 kDa (cmplx I) | O95168 | 5c | 1.5 |
| FIS1 | Fission-1 (mitochondrial outer membrane) homolog (S. cerevisiae) (cmplx I) | Q9Y3D6 | 9u | 1.5 |
| UQCRC2 | Ubiquinol-cytochrome c reductase core protein II (cmplx III) | P22695 | 32u | 1.4 |
| SDHB | Succinate dehydrogenase complex, subunit B, iron sulfur (cmplx II) | P21912 | 6u | 1.4 |
| NDUFB1 | NADH dehydrogenase (ubiquinone) 1 beta subcomplex 1, 7 kDa (cmplx I) | A0AV68 | 4u | 1.4 |
| COX7A2L | Cytochrome c oxidase, subunit VIIa, polypeptide 2 like (cmplx IV) | O14548 | 4c | 1.4 |
| AIFM1 | Apoptosis-inducing factor, mitochondrion-associated | O95831-1 | 10c | 1.4 |
| CAT | Catalase | P04040 | 24u | 1.3 |
| NDUFS5 | NADH dehydrogenase (ubiquinone) Fe-S protein 5, 15 kDa (cmplx I) | O43920 | 6u | 1.3 |
| NDUFS7 | NADH dehydrogenase (ubiquinone) Fe-S protein 7, 20 kDa (cmplx I) | O75251 | 3c | 1.3 |
| Oxidative stress | ||||
| SOD1 | Superoxide dismutase 1, soluble | P00441 | 5u | 2.1 |
| STAT3 | Signal transducer and activator of transcription 3 (acute-phase response factor) | P40763-1 | 4c | 2.1 |
| MAPK14 | Mitogen-activated protein kinase 14 | Q16539-1 | 2c | 2.0 |
| GSTM3 | Glutathione S-transferase m3 (brain) | P21266 | 3u+10c | 1.8 |
| PRDX5 | Peroxiredoxin 5 | P30044-1 | 26c | 1.8 |
| PRDX6 | Peroxiredoxin 6 | P30041 | 57u+29c | 1.7 |
| ME1 | Malic enzyme 1, NADP(+)-dependent, cytosolic | P48163 | 8u | 1.6 |
| GCLM | Glutamate-cysteine ligase, modifier subunit | P48507 | 2u | 1.5 |
| GSS | Glutathione synthetase | P48637 | 2u | 1.5 |
| SOD2 | Superoxide dismutase 2, mitochondrial | P04179 | 13c | 1.5 |
| NQO1 | NAD(P)H dehydrogenase, quinone 1 | P15559 | 2u+22c | 1.4 |
| GPX1 | Glutathione peroxidase 1 | P07203 | 2u | 1.4 |
| CAT | Catalase | P04040 | 24u | 1.3 |
| Cholesterol Biosynthesis | ||||
| HMGCS1 | 3-hydroxy-3-methylglutaryl CoA synthase 1 (soluble) | Q01581 | 4u | 2.2 |
| LSS | Lanosterol synthase (2,3-oxidosqualene-lanosterol cyclase) | P48449 | 2u | 2.0 |
| PMVK | Phosphomevalonate kinase | Q15126 | 4u | 1.7 |
| FDFT1 | Farnesyl-diphosphate farnesyltransferase 1 | P37268 | 5c | 1.7 |
| FDPS | Farnesyl diphosphate synthase | B3KMW3 | 7c | 1.6 |
| ACAT2 | Acetyl-CoA acetyltransferase | Q9BWD1 | 13u | 1.3 |
u - proteins identified by unique peptides; c - proteins identified by common peptides.