Skip to main content
. Author manuscript; available in PMC: 2013 Sep 1.
Published in final edited form as: Cell Microbiol. 2012 Jun 5;14(9):1485–1495. doi: 10.1111/j.1462-5822.2012.01815.x

Figure 1. Sca234–556 is sufficient to mediate adherence to the surface of mammalian cells.

Figure 1

A. Immunofluorescence microscopy of purified GST-fused Sca2 peptides adhered to the surface of a HeLa (left panels) or EA.hy 926 (right panels) cell monolayers. Scale bar represents 20 μm. B. Flow cytometric analysis of titration of GST-fused Sca2 peptides bound to the surface of HeLa cells. C. Graph of Mean FITC signal of data presented in Figure B as a function of protein concentration. D and E. Flow cytometric analysis of GST-fused Sca2 peptides (at 1.25 μM) bound to the surface of EA.hy cells (D) and HeLa cells (E). Twenty thousand events were recorded for all flow cytometry samples. All subscripted text refers to amino acid numbers.