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. Author manuscript; available in PMC: 2013 Sep 1.
Published in final edited form as: Cell Microbiol. 2012 May 23;14(9):1455–1473. doi: 10.1111/j.1462-5822.2012.01811.x

Figure 3. miR6 and miR10 can repress the expression of myc-VSP1267 in Giardia.

Figure 3

(A) Expression of myc-VSP1267 after introducing miR6 or/and miR10 was examined in Western blot. Synthetic miR6, miR10 or miR6+miR10 was introduced into the trophozoites, which express Tet-inducible myc-VSP1267. The protein level of myc-VSP1267 was monitored after a 16 hr incubation at 37°C. Five independent experiments were carried out. (B) The quantitative data from (A) were shown with the expression of myc-VSP1267 without miRNAs set at 1. The p-values indicated were calculated by two-tailed Student’s t-test. (C) Immuno-fluorescence assay of myc-VSP1267-expressing trophozoites after introducing miR6 or/and miR10. The same cell samples used in Western blot analysis were used for immunostaining of myc-VSP1267. The cell images were taken and the mean integrated intensity of fluorescence per cell in each image was measured using the CellProfiler program (Lamprecht et al., 2007). For each sample, at least five images were taken. (D) The quantitative integrated intensity of fluorescence per cell was analyzed. The p-values indicated were calculated by two-tailed Student’s t-test.