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. Author manuscript; available in PMC: 2013 Sep 21.
Published in final edited form as: Oncogene. 2012 May 14;32(12):1570–1579. doi: 10.1038/onc.2012.167

Figure 2. Negative feedback loop existed between miR-502 and p53.

Figure 2

(A) Ectopic expression of miR-502 in HCT116 cells decreased protein levels of p53 and p21 by western blot analysis. (B) Treating HCT116 (p53+/+) cells with 10µM nutlin-3 for 24 hours induced elevated protein levels of p53 and (C) decreased levels of endogenous miR-502 by real-time qRT-PCR (n=3, p < 0.001). (D) A 2.5 Kb gene fragment containing putative p53 binding site at 2472 bp upstream of miR-502 coding sequence was inserted into pGL3-promoter vector. Nutlin-3 treatment inhibited the luciferase activity in HCT116 (p53+/+) cells but not in HCT116 (p53−/−) cells as compared to the negative control (NC) (n=3, p < 0.001).

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