Figure 3. miR-502 inhibited autophagy in HCT116 cells.

Forty-eight hours after transfection of miR-502 and negative miRNA, HCT116 (p53+/+) and HCT116 (p53−/−) cells were treated with EBSS and lysosomal inhibitor BafA1. Western blot analysis of LC3B and p62 levels was performed after two hours (A) and 24 hours (C) and the densitometry of LC3B-II normalized to GAPDH is labeled under each lane. The fold changes in autophagic flux were shown in (B) and (D), respectively (n=3).