Fig. 4.

In the developing hindbrain knock-down of atx expression does not affect neuronal/axonal organization. Embryos were treated as described in Fig. 3 and analyzed at 48 hpf. A: Representative images of embryos after whole-mount immnuostaining with the RMO44 antibody. Images represent 2D maximum projections of stacks of 5.66 µm optical sections. Arrowheads point toward T-interneurons. The gray boxes indicate the area used to determine the number of RMO44-immuno-positive pixels. Scale bar: 100 µm. B–C: Bar graphs depicting the number of RMO44-immuno-positive T-interneurons (B) and pixels (C) in % (control = 100%). Means and standard errors of three independent experiments are shown. Student’s t-test revealed no statistically significant differences.