Table 1.
Strains and plasmids used in this study
| Strain or Plasmid | Relevant characteristic (s) | Source or reference |
|---|---|---|
| A. hydrophila SSU | CDCa | |
| SSU-Rifr | Rifr strain of A. hydrophila SSU | Laboratory stock |
| ΔqseB | qseB gene deletion mutant of A. hydrophila SSU-R strain Rifr Kmr | [10] |
| ΔahyRI | ahyRI gene deletion mutant of A. hydrophila SSU-R strain Rifr Smr Spr | [7] |
| ΔluxS | luxS mutant of A. hydrophila SSU-R strain Rifr Kmr | [8] |
| SSU (pBAD::aha0701h) | WT A. hydrophila SSU with GGDEF domain protein overproduced | [9] |
| ΔahyRI (pBAD::aha0701h) | ahyRI gene deletion mutant of A. hydrophila with GGDEF domain protein overproduced | [9] |
| ΔqseB (pBAD::aha0701h) | qseB gene deletion mutant of A. hydrophila SSU with GGDEF domain protein overproduced | This study |
| Plasmids | ||
| pBAD/Myc-HisB | vector, ara BAD promoter Apr | Invitrogen |
| pBAD:: aha0701h | GGDEF domain encoded gene of A. hydrophila cloned into pBAD/Myc-HisB ara Kmr Apr | [9] |
Abbreviations: Rif, rifampin; Km, kanamycin, Sm, streptomycin, Sp, spectinomycin,
a
Centers for Disease Control and Prevention