Table 2.
Oligonucleotides used and RT-PCR products
| Primers | Oligonucleotide sequences | Amplification of the genes | RT-PCR product (bp) |
|---|---|---|---|
|
| |||
| qseBF2 | TGCTCAAGAGCGAGGAGTTTG | qseB | 551 |
| qseBR2 | CTTCTTGCGCAGGTGGTGAAT | ||
|
| |||
| qseCF2 | ATGGAGGAGCTGTTCGATGCC | qseC | 793 |
| qseCR2 | AGCAGCATCTTCTGCAGGGAGT | ||
|
| |||
| vpsTF1 | TCAGAGATACTCCTTGGCCCA | vpsT (csgAB) | 645 |
| vpsTR1 | CGCTTCATGATCACCCCATA | ||
|
| |||
| fleNF1 | TGGTCTGCGCAAAATGCGT | fleN | 856 |
| fleNR1 | TTATTCACGGGAACCTTCCTG | ||
|
| |||
| luxSF2 | ACCTCCAAGTGGGATGCGTAT | luxS | 971 |
| luxSR2 | CGGGCCATCGAAAAAATGT | ||
|
| |||
| ahyRF1 | TATTGCATCAGCTTGGGGAA | ahyR | 781 |
| ahyRR1 | TGAAACAAGACCAACTGCTTG | ||