Figure 4.

Apoptosis modulates signaling pathways that alter gene expression, protein levels and phosphorylation status of lipogenic enzymes. EL4 cells were treated with 15 μM etoposide for up to 16 h and protein samples were obtained every 2 h. (a) Representative western blots showing of the effect of apoptosis induction on the levels of p53, Akt, p-Akt (Ser473), pS6K (Ser371), pS6K (Thr389), AMPK and p-AMPK (Thr172). Actin was used as an internal control for protein loading. (b) Expression levels of Sestrin 2 () and p21 () obtained by quantitative polymerase chain reaction (PCR). Results are expressed in relation to their initial levels (untreated controls). Actin expression levels were used to normalize the results (mean±S.E.M.; ^*P<0.01, ^**P<0.001, n=3). (c) Expression levels of ACLY (), ACC1 () and FASN () obtained by quantitative PCR. Results were normalized to the actin levels in each sample and expressed relative to the untreated control (mean±S.E.M.; ^*P<0.01, ^**P<0.001, n=3). (d) Western blot analysis of the levels of ACLY, p-ACLY (Ser454), ACC1, p-ACC1 (Ser79), FASN and ACS. Actin was used as an internal protein loading control. A representative sample is shown for each protein