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. Author manuscript; available in PMC: 2013 Aug 14.
Published in final edited form as: Cancer Cell. 2012 Aug 14;22(2):209–221. doi: 10.1016/j.ccr.2012.06.007

Figure 6. TAL1 positively regulates the MYB oncogene, which coordinately regulates TAL1 target genes.

Figure 6

(A) Gene tracks represent binding of TAL1, HEB, E2A, LMO1, GATA3, RUNX1 and CBP at the MYB gene locus in Jurkat cells. See Figure 1A legend for details. (B) Co-occupancy by the TAL1 complex at the MYB gene in multiple TALL cell samples by ChIP-PCR. See Figure 3B legend for details. (C) mRNA (top) and protein (bottom) levels of MYB after knockdown (KD) of TAL1, GATA3 and RUNX1 in Jurkat cells. The data are means ± SD of duplicate experiments; *p<0.05 and **p<0.01 by two sample, two-tailed t-test. (D) GSEA of expression changes of high-confidence TAL1 targets upon MYB knockdown. TAL1 target genes (n=238) that were significantly downregulated by TAL1 knockdown were used as a gene set. See Figure 4A legend for details. (E) Positive feed-forward loop formed by the TAL1 complex and MYB that controls the gene expression program of T-ALL cells. MYB is bound and activated by the TAL1 complex and, in turn, regulates the same set of genes. See also Figure S5.