Fig. 6.

Correlation of UGT1A1 mRNA expression with HLM glucuronidation activity. A and B, UGT1A1 v1 and UGT1A1 v2/v3 mRNA levels were assayed in 58 human liver specimens and correlated with glucuronidation activity against raloxifene (A) and bilirubin (B) in HLMs derived from the same specimens. C and D, correlations of UGT1A1 v1 (C) and UGT1A1 v2/v3 (D) expression with raloxifene-6-O-glucuronide formation are shown after stratification by UGT1A1 TATAA box genotype. RQ values were calculated for v1 and v2/v3 mRNA separately by the ΔΔCt method.