Fig. 8.

Competition of l-AMT transport by the human PCFT. CHO cells transfected with human PFCT (R2/hPCFT4 cells) or transporter-null R2 cells were incubated with 0.5 μM [³H]l-AMT at 37°C for 2 min at pH 5.5 (A), pH 6.5 (B), or pH 6.8 (C), and the transport of [³H]l-AMT competed with 0.5% DMSO (vehicle for compound 1; Wang et al., 2010), 10 μM l-AMT, 10 or 30 μM d-AMT, 10 μM PMX, or 10 μM compound 1. A no-addition (NA) incubation served as a negative competition control. Values are averages ± S.D. (n = 4).