Fig. 5.

Glucuronidation of C-1305 (A) and C-1311 (B) by human stomach and intestine microsomes. Microsomes (20 μg) were prepared from the mucosa of stomach (ST), duodenum (D), four segments of small intestine (S1–S4, proximal to distal), and colon (C) from four different donors. The substrate and UDP-GlcUA concentrations were 0.1 and 3 mM, respectively, and the reactions were incubated for 60 min. For each box and whisker plot, the line bisecting the box is the median value, the upper and lower limits of the box are the 25th and 75th percentile values, respectively, and the error bars (whiskers) extend to the lowest and highest values. ***, p < 0.001, indicates a significant effect of donor and fragment with significant interaction (two-way analysis of variance with Bonferroni posttest).