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. Author manuscript; available in PMC: 2013 Oct 1.
Published in final edited form as: Biomaterials. 2012 Jul 17;33(30):7497–7507. doi: 10.1016/j.biomaterials.2012.06.099

Figure 6.

Figure 6

Effect of serial passaging on BMMΦ proliferation. Fluorescence and color images of: A) DAPI and Ki-67, and B) SA beta-gal staining, respectively, in primary BMMΦ cultures with increasing passage numbers. Graphical representation of: C) percent positive cells for Ki-67 markers, and D) SA beta-gal (from fluorescent senescence assay) with increasing passaging in BMMΦs. These data show no dramatic increasing or decreasing trends in senescence with increased passaging. One-way ANOVA with post-hoc Dunnett Multiple Comparisons Test was applied, comparing all passages to the passage 1 condition. Significance is noted as p<0.05 * and p<0.001 **.