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. Author manuscript; available in PMC: 2012 Aug 19.
Published in final edited form as: Mol Microbiol. 2009 Feb 10;71(6):1574–1590. doi: 10.1111/j.1365-2958.2009.06623.x

Fig. 4. Assessment of the FHA RGD triplet in B. bronchiseptica pathogenesis.

Fig. 4

A. Wistar rats were inoculated intranasally with 10 μl PBS containing 1000 cfu of B. bronchiseptica (strains indicated across the bottom) and the number of cfu recovered from the nasal cavity (NC) and 1 cm trachea determined at days 14 and 28 post inoculation. Each circle represents the number of cfu recovered from a single animal. The horizontal line shows the geometric mean for each group. The dashed line represents the lower limit of detection. y-axis is log scale. Asterisks indicate a statistically significant difference compared with RB50 (P < 0.05).

B. BALB/c mice were inoculated intranasally with 50 μl PBS containing 5 × 105 cfu of B. bronchiseptica (strains indicated across the bottom) and the number of cfu in the right lung lobes was determined 60 min (day 0), 3 and 11 days post inoculation. Each circle represents the number of cfu recovered from a single animal. The horizontal line shows the geometric mean for each group. The dashed line represents the lower limit of detection. For RBX9, the means at day 3 for moribund (~109) and healthy (~106) animals were calculated separately as indicated. y-axis is log scale. Asterisks indicate a statistically significant difference compared with RB50 (P < 0.05).

C and D. SCID/Bg mice were inoculated intranasally with 50 μl PBS containing 5 × 105 (for C) or 10 μl containing 1000 (for D) cfu of RB50 (solid line) or RB50RAE (dashed line) and the number of survivors plotted over time. The mean time to death was not significantly different between RB50 and RB50RAE for either experiment, as determined by the Log Rank (Mantel-Cox) test. RB50 and RBX9 have been compared in these models many times with similar results. Each type of animal experiment that included the RB50RAE strain was performed once and not repeated in the interest of minimizing the number of animals used because no difference between RB50RAE and RB50 was detected in any of the experiments.