Fig. 4.
Time course of effects of 1-benzyl-I3C on expression of G1 cell cycle components. Upper panel: Human MCF-7 breast cancer cells were treated with 0.2 μM 1-benzyl-I3C or the DMSO vehicle control for 0, 24, 48 or 72 hours followed by western blot analysis of the indicated G1 cell cycle components. Actin protein levels were used a gel loading control. Lower panel: The level of observed cell cycle protein in 1-benzyl-I3C treated cells was compared to control cells and quantified at each time point by densitometric analysis of the western blot.