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Dose-dependent effects of 1-benzyl-I3C and I3C on expression of estrogen receptor-alpha protein. MCF-7 cells were treated with indicated concentrations of 1-benzyl-I3C or I3C for 24 hours, and the level of estrogen receptor-alpha (ERα) determined by western blot analysis of electrophoretically fractionated total cell extracts. Western blot analysis of actin levels was used as a gel loading control.
