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. Author manuscript; available in PMC: 2012 Aug 19.
Published in final edited form as: Exp Cell Res. 2007 Jul 19;313(15):3261–3275. doi: 10.1016/j.yexcr.2007.07.009

Fig. 5.

Fig. 5

Knockdown of importin-alpha using RNA interference. Con8 cells were reverse transfected with 150 nM siRNA specific to importin-alpha or 150 nM of nonspecific (control) siRNA at the time of plating. Cells were then treated with or without 1 uM dexamethasone for 24 hours. Cell lysates were separated on SDS-PAGE and immunoblotted with anti-importin-alpha or actin specific antibodies as described in the Materials and Methods section.