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. Author manuscript; available in PMC: 2012 Aug 19.
Published in final edited form as: Exp Cell Res. 2007 Jul 19;313(15):3261–3275. doi: 10.1016/j.yexcr.2007.07.009

Fig. 6.

Fig. 6

Effects of RNAi knock down of importin-alpha on the localization of Sgk and ERK. Con8 cells were reverse transfected with 150 nM siRNA specific to importin-alpha or 150 nM of nonspecific (control) siRNA at the time of plating. Cells cultured in 10% serum were then treated with or without 1 uM dexamethasone (Dex) for 24 hours. Panel A: The subcellular distribution of Sgk was examined by indirect immunofluorescence microscopy using affinity-purified rabbit polyclonal antibodies to Sgk followed by FITC-conjugated goat anti-rabbit secondary antibodies. Panel B: Erk1/Erk2 were detected using monoclonal antibodies that specifically recognized both MAPK family members followed by a rhodamine-conjugated anti-rabbit secondary antibody employed to selectively visualize this protein kinase. DNA was stained to visualize the nucleus using DAPI.