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. Author manuscript; available in PMC: 2012 Aug 19.
Published in final edited form as: Exp Cell Res. 2007 Jul 19;313(15):3261–3275. doi: 10.1016/j.yexcr.2007.07.009

Fig. 7.

Fig. 7

Co-immunoprecipitation of Sgk with Erk/MAPK. Panel A: Cells were transiently transfected with a wild-type Sgk expression vector and then treated with the indicated combinations of 10% serum and/or 1 μM dexamethasone for 24 hours. Cell extracts were immunoprecipitated with antibodies directed against either Erk1/Erk2 or to actin, the immunoprecipitated material electrophoretically fractionated and probed for the presence of Sgk. Panel B: Cells were transfected with a wild-type (WT) or the kinase dead (K127M) forms of Sgk or with an empty expression vector (V). Erk1/Erk2 was immunoprecipitated from the cell extracts, electrophoretically fractionated and western blots probed for the presence of Sgk.