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. Author manuscript; available in PMC: 2012 Aug 19.
Published in final edited form as: Exp Cell Res. 2007 Jul 19;313(15):3261–3275. doi: 10.1016/j.yexcr.2007.07.009

Fig. 8.

Fig. 8

Effects of serum and glucocorticoids on production of the hyperphosphorylated forms of Sgk and Erk/MAPK. Serum-treated cells were incubated with 1 μM dexamethasone and at the indicated time points, total cell extracts were electrophoretically fractionated. Western blots were probed for production of activated MAPK (top panel) or total Erk1/Erk2 (middle panel) using the appropriate antibodies. Cells were treated with 10% serum or 1 μM dexamethasone (Dex) in presence or absence of 50 μM LY294002, which inhibits PI 3-kinase activity, for 24 hours. A control culture was maintained in the absence of either stimuli (0 hr). Cell extracts were electrophoretically fractionated and probed for Sgk using affinity purified anti-Sgk antibodies. The hyperphosphorylated and hypophosphorylated Sgk species are designated with arrows.