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. Author manuscript; available in PMC: 2012 Aug 19.
Published in final edited form as: Exp Cell Res. 2007 Jul 19;313(15):3261–3275. doi: 10.1016/j.yexcr.2007.07.009

Fig. 9.

Fig. 9

Effects of the PD 98095 MEK inhibitor on the subcellular localization of Sgk and Erk/MAPK. Con8 cells were treated with 10% serum for 18 hours and 50 uM PD 98095 for the last 8 hours. The subcellular distribution of Sgk and Erk/MAPK was examined by indirect immunofluorescence microscopy using affinity-purified rabbit polyclonal antibodies to Sgk (upper panel) or monoclonal anti-Erk 1 and Erk 2 antibodies (lower panel) as the primary antibodies respectively. The secondary antibodies were FITC-conjugated goat anti-rabbit and rhodamine-conjugated goat-anit-mouse, respectively.