Figure 6.

Transcription using the unnatural s–y pair. (a) Scheme of the transcription experiments and 2D-TLC for nucleotide composition analyses of transcripts. (b) The coupled transcription–translation system involving the s–y pair. The DNA template containing a CTs sequence and the 3-chlorotyrosine-charged tRNA were prepared separately and added to the transcription-coupled translation system. The yAG codon–CUs anticodon interaction was used for the site-specific incorporation of 3-chlorotyrosine at position 32 of the human Ras protein.