Skip to main content
. Author manuscript; available in PMC: 2012 Dec 22.
Published in final edited form as: Cell. 2012 Jun 14;149(7):1447–1460. doi: 10.1016/j.cell.2012.03.052

Figure 1. Design of Chromatin in vivo Assay at Oct4 (CiA:Oct4) ES cell line and mouse.

Figure 1

(A) CIP allows direct recruitment, washout, co-occupancy, and order-of-addition experiments. (B) The CIA:Oct4 mouse contains one modified Oct4 allele harboring two arrays of DNA binding sites (12XZFHD1 and 5XGal4) in the promoter region upstream of an in-frame EGFP reporter. Distribution of histone modifications at the Oct4 locus in murine ES cells and brain tissue (Mikkelsen et al., 2007) reveals the distinct chromatin substrates for CiA modulation.