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Mass spectrometric sequence coverage of the β3 subunit of the GABAA receptor. Five micrograms of Sf9 cell membranes expressing His8-β3 homomeric GABAA receptors were photolabeled with 15 μM 6-AziP. The receptors were purified with a nickel affinity column and were analyzed through mass spectrometry after Lys-C/trypsin or Lys-C/chymotrypsin digestion. Ninety percent of the peptide sequence was detected, as shown in red. Frames indicate the TMDs of the protein.
