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. 2012 Sep;82(3):500–509. doi: 10.1124/mol.112.079293

Fig. 2.

Fig. 2.

Ouabain and digoxin selectively increased RGS2 protein expression. A and B, ouabain (A) and digoxin (B) increased RGS2 protein expression in stably transfected HEK-293 cells, in a concentration-dependent manner, as demonstrated with the PathHunter ProLabel assay. The effect was selective over closely related RGS4, because neither compound increased RGS4 protein expression in the same assay system. C, Western blotting showed increased RGS2 protein expression with ouabain and digoxin in transiently transfected HEK-293T cells. D, ouabain and digoxin increased endogenous RGS2 protein expression in rat primary aortic VSMCs, as demonstrated through Western blotting. All treatments were overnight. **, p < 0.01; ***, p < 0.001, using one-way analysis of variance with Bonferroni's post hoc test for multiple comparisons.